Ca2+ waves coordinate purinergic receptor–evoked integrin activation and polarization

Bye, A. P. ORCID: https://orcid.org/0000-0002-2061-2253, Gibbins, J. M. ORCID: https://orcid.org/0000-0002-0372-5352 and Mahaut-Smith, M. P. (2020) Ca2+ waves coordinate purinergic receptor–evoked integrin activation and polarization. Science Signaling, 13 (615). eaav7354. ISSN 1945-0877 doi: 10.1126/scisignal.aav7354

Abstract/Summary

The integrin GPIIb/IIIa is highly abundant on the surface of platelets and can be activated by intracellular Ca2+ signaling in an “inside-out” manner to bind to the adhesive ligand fibrinogen. Bye et al. imaged intracellular Ca2+ signaling and fibrinogen binding events in primary rat megakaryocytes activated through the ADP-stimulated receptors P2Y1 and P2Y12. The authors found that signaling by both receptors was required for full integrin activation, which depended on P2Y1-stimulated Ca2+ signaling and P2Y12-stimulated activation of the kinase PI3K. In addition, fibrinogen binding became polarized in these cells in a manner dependent on the direction of ADP-stimulated Ca2+ waves.

Item Type	Article
URI	https://reading-pure-test.eprints-hosting.org/id/eprint/88548
Identification Number/DOI	10.1126/scisignal.aav7354
Refereed	Yes
Divisions	Life Sciences > School of Biological Sciences > Biomedical Sciences Central Services
Download/View statistics	View download statistics for this item