Precise quantification of platelet proteins and their phosphorylation states

Mazet, F. and Fry, M. J. (2018) Precise quantification of platelet proteins and their phosphorylation states. In: Gibbins, J. M. and Mahaut-Smith, M. (eds.) Platelets and Megakaryocytes: Volume 4, Advanced Protocols and Perspectives:. Humana Press, New York, pp. 113-125. ISBN 9781493985845 doi: 10.1007/978-1-4939-8585-2_7

Abstract/Summary

Systems biology and modeling approaches require quantitative data-rich temporal experiments to better understand the dynamics and regulation of the components of the signaling pathways that governs cell biology and physiology. Here we present a modified Western blotting method to rapidly analyze and accurately quantify protein copy number, and their respective phosphorylation states at specific sites over detailed time courses.