From Loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes

Barr, J., Prieto Otoya, T. D., Cardin, C. ORCID: https://orcid.org/0000-0002-2556-9995 and Cadoni, E. (2026) From Loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes. RSC Chemical Biology, 7 (1). pp. 44-48. ISSN 2633-0679 doi: 10.1039/d5cb00247h

Abstract/Summary

We report the use of 5-furyl-2′-deoxyuridine (5FU) as a fluorescent probe to distinguish ligand binding to distinct G-quadruplex tetrads. Site-specific incorporation of 5FU into T95-2T at the capping regions enabled discrimination of peptide-binding events via fluorescence turn-on, providing insights into tetrad preference and binding affinities using the 5FU-G4/RHAU peptide system.

Item Type	Article
URI	https://reading-pure-test.eprints-hosting.org/id/eprint/127282
Identification Number/DOI	10.1039/d5cb00247h
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > Department of Chemistry Central Services
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