Isolation and characterization of extracellular vesicles derived from human umbilical cord mesenchymal stem cells

Ridzuan, N., Widera, D. ORCID: https://orcid.org/0000-0003-1686-130X and Yahaya, B. H. (2022) Isolation and characterization of extracellular vesicles derived from human umbilical cord mesenchymal stem cells. In: Kannan, N. and Beer, P. (eds.) Stem Cell Assays:. Methods in Molecular Biology. Springer, pp. 271-280. ISBN 9781071619797 doi: 10.1007/978-1-0716-1979-7_18 (ISSN 1940-6029)

Abstract/Summary

The safety and efficacy of mesenchymal stem cells/marrow stromal cells (MSC) have been widely studied. Since they are hypoimmunogenic, MSC can escape immune recognition, thus making them an attractive tool in clinical settings beyond autologous cell-based therapy. Paracrine factors including extracellular vesicles (EVs) released by MSC play a significant role in exerting therapeutic effects of MSC. Since their first discovery, MSC-EVs have been widely studied in an attempt to tackle the mechanisms of their therapeutic effects in various disease models. However, currently there are no standard methods to isolate EVs. Here, we describe a differential centrifugation-based protocol for isolation of EVs derived from human umbilical cord MSC (huc-MSC). In addition, the protocol describes methods for characterization of the EVs using transmission electron microscope, Western blot, and nanoparticle tracking analysis.

Item Type	Book or Report Section
URI	https://reading-pure-test.eprints-hosting.org/id/eprint/105193
Identification Number/DOI	10.1007/978-1-0716-1979-7_18
Refereed	Yes
Divisions	Life Sciences > School of Chemistry, Food and Pharmacy > School of Pharmacy
Publisher	Springer
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